Articles

Cytomics of the tumour microenvironment: therapeutic targeting (keynote lecture)

Authors: Paul Smith

The effect of aged erythrocytes and erythrocyte-derived microparticles on lymphomatous and healthy B cells

Authors: Caitlin Hughes, Mark A Vickers, Robert N Barker and Lindsay S Hall

Translating research flow cytometry assays for characterization of cellular therapeutics

Authors: John DM Campbell

Cell therapies for joint repair

Authors: Cosimo De Bari

TGF-β2 mediated secretion of sCTLA-4 from regulatory T cells

Authors: R Khanolkar, S Rajpara, F Muller, I Depasquale, L Lawson, RN Barker, M Nicolson, AD Ormerod and FJ Ward

Quality in clinical cytometry

Authors: David WL Wilson

Characterisation of the murine C-type lectin receptor CLECSF8 (MCL) reveals its expression on cells of the monocyte/neutrophil lineages and an inter-dependence with Mincle, but not Dectin-2

Authors: Bernhard Kerscher, Gillian J Wilson, Delyth M Reid, Sho Yamasaki, Janet A Willment and Gordon D Brown

FACS for Fungi: revealing population heterogeneity among fungal pathogens via flow cytometry

Authors: Elizabeth R Ballou and Alistair JP Brown

CCL19-sorted mature dendritic cells have enhanced lymph node migratory capacity and function

Authors: Michelle L Le Brocq, Alasdair R Fraser, Gerard J Graham and John D M Campbell

Deletion of myeloid-PTP1B decreases MHC Class I expression and peptide presentation through an IL-10 dependent mechanism in response to LPS challenge

Authors: Samantha Le Sommer, Cristina Martin-Granados and Mirela Delibegovic

Introduction and welcome

Authors: Raif Yuecel

Role of the NLRP3 inflammasome in the transient release of IL-1β induced by monosodium urate crystals in human fibroblast-like synoviocytes

To investigate whether monosodium urate (MSU) crystals induce interleukin (IL)-1β in human fibroblast-like synoviocytes (FLS), and whether the NLRP3 inflammasome is involved in the inflammatory mechanism.

Authors: Shu-cong Zheng, Xiao-xia Zhu, Yu Xue, Li-hong Zhang, He-jian Zou, Jian-hua Qiu and Qiong Liu

Adipokine gene expression in peripheral blood of adult and juvenile dermatomyositis patients and their relation to clinical parameters and disease activity measures

Recently adipokines have been implicated in the regulation of immune and inflammatory responses in autoimmune disease. To investigate the role of adipokines in adult and pediatric patients with newly diagnosed...

Authors: Jeannette M Olazagasti, Molly Hein, Cynthia S Crowson, Consuelo Lopez de Padilla, Erik Peterson, Emily C Baechler and Ann M Reed

Reduced expression of peroxisome proliferator-activated receptor alpha in BAL and blood T cells of non-löfgren’s sarcoidosis patients

Sarcoidosis is a granulomatous disease affecting in particular the lungs. The peroxisome proliferator-activated receptors (PPARs) play important regulatory roles in inflammation. The aim of this study was to g...

Authors: Muntasir Abo Al Hayja, Anders Eklund, Johan Grunewald and Jan Wahlström

Valproic acid attenuates intercellular adhesion molecule-1 and E-selectin through a chemokine ligand 5 dependent mechanism and subarachnoid hemorrhage induced vasospasm in a rat model

Up-regulation of regulated upon activation, normal T-cell expressed and secreted (RANTES/CCL5) and adhesion molecules is observed in the serum of animals following experimental subarachnoid hemorrhage (SAH). T...

Authors: Chih-Zen Chang, Shu-Chuan Wu, Chih-Lung Lin and Aij-Lie Kwan

Chronic Kidney Disease is associated with an increase of Intimal Dendritic cells in a comparative autopsy study

Chronic Kidney Disease (CKD) and inflammation are risk factors for atherosclerotic vascular disease (ASVD). In inflammatory conditions, Nuclear Factor-κB (NF-κB) is frequently activated and it has been detecte...

Authors: Miguel Hueso, Joan Torras, Marta Carrera, August Vidal, Estanis Navarro and Josep Grinyó

Functional activity of peripheral blood eosinophils in allergen-induced late-phase airway inflammation in asthma patients

We aimed to investigate peripheral blood eosinophil chemotaxis, generation of spontaneous reactive oxygen species (ROS), and apoptosis in patients with allergic asthma after bronchial allergen challenge.

Authors: Simona Lavinskiene, Kestutis Malakauskas, Jolanta Jeroch, Deimante Hoppenot and Raimundas Sakalauskas

Expression of hedgehog signal pathway in articular cartilage is associated with the severity of cartilage damage in rats with adjuvant-induced arthritis

Cartilage damage is a crucial step in rheumatoid arthritis (RA) disease progress while its molecular mechanisms are not fully understood. Here we investigated the expression of hedgehog (Hh) signal pathway in ...

Authors: Rong Li, Li Cai, Cheng-mu Hu, Ting-ni Wu and Jun Li

The protective effects of chronic intermittent hypobaric hypoxia pretreatment against collagen-induced arthritis in rats

To explore the immunological mechanisms underlying the effect of chronic intermittent hypobaric hypoxia (CIHH) pretreatment on collagen-induced arthritis (CIA) in rat.

Authors: Min Shi, Fang Cui, Ai-Jing Liu, Hui-Jie Ma, Ming Cheng, Shu-Xia Song, Fang Yuan, De-Pei Li and Yi Zhang

TNF-alpha and IL-6 inhibit apolipoprotein A-IV production induced by linoleic acid in human intestinal Caco2 cells

Apolipoprotein A-IV (apoA-IV) is a protein mainly synthesized by enterocytes in the intestine. Its gene expression is suppressed during fasting and stimulated during active fat absorption. Chronic feeding of a...

Authors: Xiaoming Li, Min Xu, Min Liu, Yong Ji and Zongfang Li

Association of serum TNF-α, IL-8 and free light chain with HLA-DR B alleles expression in pulmonary and extra-pulmonary sarcoidosis

Sarcoidosis is a systemic disease of unknown etiology characterized histologically by the observation of non-caseating granulomas and several immunological abnormalities. Sarcoidosis is a multi-organ disorder ...

Authors: Esmaeil Mortaz, Hale Abdoli Sereshki, Atefeh Abedini, Arda Kiani, Mehdi Mirsaeidi, Dina Soroush, Johan Garssen, Aliakbar Velayati, Frank A Redegeld and Ian M Adcock

Neutrophil-to-lymphocyte ratio, calprotectin and YKL-40 in patients with chronic obstructive pulmonary disease: correlations and 5-year mortality – a cohort study

Chronic obstructive pulmonary disease (COPD) is characterized by chronic inflammation and progressive decline in pulmonary function. Neutrophil-to-lymphocyte ratio (NLR), YKL-40 and calprotectin are biomarkers...

Authors: Allan Klitgaard Sørensen, Dennis Back Holmgaard, Lone Hagens Mygind, Julia Johansen and Court Pedersen

Crude extract of hydatid laminated layer from Echinococcus granulosus cyst attenuates mucosal intestinal damage and inflammatory responses in Dextran Sulfate Sodium induced colitis in mice

Inflammatory bowel disease is an immunologically mediated disease. Notably, it is less common in countries where there is a greater risk of exposure to helminths. In our study, we examined the modulatory effec...

Authors: Imene Soufli, Ryma Toumi, Hayet Rafa, Manel Amri, Moussa Labsi, Lila Khelifi, Ferdinando Nicoletti and Chafia Touil-Boukoffa

MicroRNA-181b regulates endotoxin tolerance by targeting IL-6 in macrophage RAW264.7 cells

Interleukin 6 (IL-6) is a major pro-inflammatory cytokine and dysregulation of IL-6 is relevant to many inflammatory diseases. Endotoxin induced tolerance of IL-6 is an important mechanism to avoid the excessi...

Authors: Wenjun Zhang, Xiaojun Shen, Luyang Xie, Maoping Chu and Yanmei Ma

Anti-inflammatory effects of an injectable copolymer of fatty acids (Ara 3000 beta®) in joint diseases

In inflammatory joint disease, such as osteoarthritis or arthritis, there is an increased level of pro-inflammatory cytokines, such as interleukin-1β. These cytokines stimulate the expression and release of ma...

Authors: Catherine Baugé, Eva Lhuissier, Nicolas Girard, Céline Quesnelle, Gérard Ewert and Karim Boumediene

Human parainfluenza virus type 3 (HPIV3) induces production of IFNγ and RANTES in human nasal epithelial cells (HNECs)

Human parainfluenza virus type 3 (HPIV3), while infecting lower airway epithelial cells induces pneumonia and bronchiolitis in infants and children, and may lead to asthma exacerbations in children and adults....

Authors: Anna Lewandowska-Polak, Małgorzata Brauncajs, Edyta Paradowska, Marzanna Jarzębska, Marcin Kurowski, Sylwia Moskwa, Zbigniew J Leśnikowski and Marek L Kowalski

Conjugated linoleic acid induces an atheroprotective macrophage MΦ2 phenotype and limits foam cell formation

Atherosclerosis, the underlying cause of heart attack and strokes, is a progresive dyslipidemic and inflammatory disease where monocyte-derived macrophage cells play a pivotal role. Although most of the mechan...

Authors: Monica de Gaetano, Kawthar Alghamdi, Simone Marcone and Orina Belton

Portrait of inflammatory response to ionizing radiation treatment

Ionizing radiation (IR) activates both pro-and anti-proliferative signal pathways producing an imbalance in cell fate decision. IR is able to regulate several genes and factors involved in cell-cycle progressi...

Authors: Federica Maria Di Maggio, Luigi Minafra, Giusi Irma Forte, Francesco Paolo Cammarata, Domenico Lio, Cristina Messa, Maria Carla Gilardi and Valentina Bravatà

Orphan nuclear receptor NR4A2 induces transcription of the immunomodulatory peptide hormone prolactin

Nuclear receptor 4A2 (NR4A2) is an orphan nuclear receptor and constitutively active transcription factor expressed at elevated levels in inflamed joint tissues from patients with arthritis. Inflammatory media...

Authors: Joseph M McCoy, Dana E Walkenhorst, Keegan S McCauley, Hiba Elaasar, Jordan R Everett and Kimberlee S Mix

Inflammation contributes to NKX3.1 loss and augments DNA damage but does not alter the DNA damage response via increased SIRT1 expression

The oxidative stress response is a cellular defense mechanism that protects cells from oxidative damage and cancer development. The exact molecular mechanism by which reactive oxygen species (ROS) contribute t...

Authors: Bilge Debelec-Butuner, Nursah Ertunc and Kemal Sami Korkmaz

Silencing collapsin response mediator protein-2 reprograms macrophage phenotype and improves infarct healing in experimental myocardial infarction model

Delayed M1 toward M2 macrophage phenotype transition is considered one of the major causes for the impaired healing after myocardial infarction (MI). While searching for molecules that modulate M1 and M2 macro...

Authors: Long-Shu Zhou, Guo-Long Zhao, Qiang Liu, Shu-Cai Jiang, Yun Wang and Dong-Mei Zhang

Antioxidant polyphenol-rich extracts from the medicinal plants Antirhea borbonica, Doratoxylon apetalum and Gouania mauritiana protect 3T3-L1 preadipocytes against H₂O₂, TNFα and LPS inflammatory mediators by regulating the expression of superoxide dismutase and NF-κB genes

Adipose cells responsible for fat storage are the targets of reactive oxygen species (ROS) like H₂O₂ and pro-inflammatory agents including TNFα and LPS. Such mediators contribute to oxidative stress and alter inf...

Authors: Méry Marimoutou, Fanny Le Sage, Jacqueline Smadja, Christian Lefebvre d’Hellencourt, Marie-Paule Gonthier and Christine Robert-Da Silva

Targeting CD226/DNAX accessory molecule-1 (DNAM-1) in collagen-induced arthritis mouse models

Genetic studies have pointed out that CD226 variants, encoding DNAM-1, could be associated with susceptibility to rheumatoid arthritis. Therefore, we aimed to determine the influence of DNAM-1 on the development ...

Authors: Muriel Elhai, Gilles Chiocchia, Carmen Marchiol, Franck Lager, Gilles Renault, Marco Colonna, Guenter Bernhardt, Yannick Allanore and Jérôme Avouac

Expression of BMP4 in myocardium and vascular tissue of obese mice

Obesity is regarded as a risk factor for cardiovascular disease. Bone morphogenetic protein 4 (BMP4) is a proinflammatory and profibrotic factor, and the reduced expression of this molecule in obese mice seems...

Authors: Ting Wu, Qiu-Yang Ling, Cheng Zhong, Tian-Xiao Wang, Lu-Lu Wang, Xiao-Ying Wang, Zhao-Liang Su and Gang-Jun Zong

Knockdown of MVK does not lead to changes in NALP3 expression or activation

Mutations in the Mevalonate Kinase gene (MVK) are causes of a rare autoinflammatory disease: Mevalonate Kinase Deficiency and its more acute manifestation, Mevalonic Aciduria. The latter is characterized, among o...

Authors: Fulvio Celsi, Elisa Piscianz, Maurizio Romano and Sergio Crovella

Effect of Sulforaphane on NOD2 via NF-κB: implications for Crohn’s disease

Sulforaphane has well established anti-cancer properties and more recently anti-inflammatory properties have also been determined. Sulforaphane has been shown to inhibit PRR-mediated pro-inflammatory signallin...

Authors: Danielle L Folkard, Gareth Marlow, Richard F Mithen and Lynnette R Ferguson

Cellular cytokine and chemokine responses to parasite antigens and fungus and mite allergens in children co-infected with helminthes and protozoa parasites

In sub-Saharan Africa poly-parasite infections are frequently observed in children, and with poly-parasitism modulating immune mechanisms, mediated by cytokines and chemokines, are required to prevent overwhel...

Authors: Jana Hegewald, Richard G Gantin, Christian J Lechner, Xiangsheng Huang, Abram Agosssou, Yvon F Agbeko, Peter T Soboslay and Carsten Köhler

Antioxidant and anti-inflammatory activity of Ocimum labiatum extract and isolated labdane diterpenoid

Plants from the genus Ocimum are used as folk medicine for treating various diseases including inflammatory and immune-related diseases. Numerous reports have suggested plant extracts and their constituents as po...

Authors: Petrina Kapewangolo, Justin J Omolo, Ronel Bruwer, Pascaline Fonteh and Debra Meyer

Interleukin-1β regulates the expression of glucocorticoid receptor isoforms in nasal polyps in vitro via p38 MAPK and JNK signal transduction pathways

To explore the upstream signal transduction mechanisms responsible for the imbalanced expression of glucocorticoid receptor (GR) isoforms in chronic rhinosinusitis (CRS) mucosa.

Authors: Zhenlin Wang, Peng Li, Qiuhang Zhang, Haili Lv, Junqi Liu and Jinyuan Si

Anti-inflammatory effects of adenosine N1-oxide

Adenosine is a potent endogenous anti-inflammatory and immunoregulatory molecule. Despite its promise, adenosine’s extremely short half-life in blood limits its clinical application. Here, we examined adenosin...

Authors: Keizo Kohno, Emiko Ohashi, Osamu Sano, Hajime Kusano, Toshio Kunikata, Norie Arai, Toshiharu Hanaya, Toshio Kawata, Tomoyuki Nishimoto and Shigeharu Fukuda

Milk-derived bioactive peptides inhibit human endothelial-monocyte interactions via PPAR-γ dependent regulation of NF-κB

Milk-derived bioactive peptides retain many biological properties and have therapeutic effects in cardiovascular disorders such as atherosclerosis. Under inflammatory conditions the expression of endothelial c...

Authors: Simone Marcone, Karen Haughton, Paul J Simpson, Orina Belton and Desmond J Fitzgerald

The histone deacetylase Hdac1 regulates inflammatory signalling in intestinal epithelial cells

It has recently been found that both nuclear epithelial-expressed histone deacetylases Hdac1 and Hdac2 are important to insure intestinal homeostasis and control the mucosal inflammatory response in vivo. In addi...

Authors: Alexis Gonneaud, Julie Moore Gagné, Naomie Turgeon and Claude Asselin

The combination of red palm oil and rooibos show anti-inflammatory effects in rats

Red palm oil (RPO) and rooibos have been shown to exhibit cardioprotective properties. RPO is rich in essential fatty acids and fat soluble antioxidants while rooibos contains polyphenolic compounds with a uni...

Authors: Emma Katengua-Thamahane, Jeanine L Marnewick, Olawale R Ajuwon, Novel N Chegou, Gergő Szűcs, Péter Ferdinandy, Tamás Csont, Csaba Csonka and Jacques Van Rooyen

Lipopolysaccharide responsiveness in vocal fold fibroblasts

Vocal fold fibroblast’s (VFF) strategic location in the lamina propria and their ability to respond to external stimuli by producing inflammatory molecules suggest their possible direct involvement in innate i...

Authors: Suzanne N King, Craig M Berchtold and Susan L Thibeault

miRNA-146 negatively regulates the production of pro-inflammatory cytokines via NF-κB signalling in human gingival fibroblasts

In human gingival fibroblasts (HGFs), TLR4 recognises Pathogen-associated molecular patterns (PAMPs), such as LPS, and subsequently activates downstream signals that lead to the production of pro-inflammatory ...

Authors: Yu-feng Xie, Rong Shu, Shao-yun Jiang, Zhong-chen Song, Qiu-man Guo, Jia-chen Dong and Zhi-kai Lin

Attenuating effect of Ginsenoside Rb1 on LPS-induced lung injury in rats

Sepsis causes neutrophil sequestration in the lung which leads to acute lung injury (ALI). Radix Ginseng (RG), a traditional herb used as herbal remedy in eastern Asia for thousands of years, which has been tr...

Authors: Qing Yuan, Yan-wen Jiang, Ting-ting Ma, Qiu-hong Fang and Lei Pan

Over-expression of TLR4-CD14, pro-inflammatory cytokines, metabolic markers and NEFAs in obese non-diabetic Mexicans

Obesity is the world’s most important public health problem. Adipose tissue contributes significantly to increase pro-inflammatory mediators whose cascade begins with the union of TLR4 to its microbial ligands...

Authors: Cesar Octavio De Loera-Rodriguez, Vidal Delgado-Rizo, Anabell Alvarado-Navarro, Juan Manuel Agraz-Cibrian, Jorge E Segura-Ortega and Mary Fafutis-Morris

Positive allosteric modulation of the adenosine A_2a receptor attenuates inflammation

Adenosine is produced at high levels at inflamed sites as a by-product of cellular activation and breakdown. Adenosine mediates its anti-inflammatory activity primarily through the adenosine A_2a receptor (A_2aR), ...

Authors: Ajith A Welihinda and Edward P Amento

Upregulation of ICAM-1 in diabetic rats after transient forebrain ischemia and reperfusion injury

Hyperglycemia exacerbates brain damage caused by cerebral ischemia. Neuroinflammation may play a role in mediating such enhanced damage. The objectives of this study were to examine the mRNA and protein levels...

Authors: Li Jing, Jian-Gang Wang, Jian-Zhong Zhang, Cai-Xia Cao, Yue Chang, Jian-Da Dong, Feng-Ying Guo and P Andy Li

Gene expression of catabolic inflammatory cytokines peak before anabolic inflammatory cytokines after ACL injury in a preclinical model

The response of the joint to anterior cruciate ligament (ACL) injury has not been fully characterized. In particular, the characterization of both catabolic factors, including interleukin-6 (IL-6), interleukin...

Authors: Carla M Haslauer, Benedikt L Proffen, Victor M Johnson, Adele Hill and Martha M Murray