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Fig. 4 | Journal of Inflammation

Fig. 4

From: PKM2/STAT1-mediated PD-L1 upregulation on neutrophils during sepsis promotes neutrophil organ accumulation by serving an anti-apoptotic role

Fig. 4

PKM2 expression and nuclear translocation inhibit dHL-60 apoptosis under LPS stimulation. dHL-60s were subjected to LPS treatment, pretreatment with shikonin/DASA-58 before LPS stimulation, or shikonin/DASA-58 treatment alone. A-D dHL-60s were stained by annexin V/PI and assessed by flow cytometry. A, C Representative flow cytometry images of dHL-60 apoptosis. B, D Histograms of the apoptosis rates of dHL-60s subjected to different treatments (n = 3–4). E-J Levels of apoptosis-related proteins were determined by Western blotting. Hsp90 was used as the loading control. The blot is representative of three independent experiments. One-way ANOVA was performed. Data are presented as the mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001

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