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Fig. 4 | Journal of Inflammation

Fig. 4

From: First trimester human umbilical cord perivascular cells (HUCPVC) modulate the kynurenine pathway and glutamate neurotransmission in an LPS-induced mouse model of neuroinflammation

Fig. 4

HUCPVC rescues the LPS-induced decline of glutamate transporter in the brain

Representative images showing double staining of astrocyte marker glial fibrillary acidic protein (GFAP, red) and excitatory amino acid transporter 2 (EAAT2, green) in the cerebral cortex region (A,B,C) and dentate gyrus region of the hippocampus (D,E,F) in the brain cryosections of control (A,D), LPS (B,E), and LPS + HUCPVC (C,F) groups. Scale bar = 50 μm. Immunohistochemistry data for cortex (G) and hippocampus (H) represented as the ratio of mean percent area of EAAT2 and the corresponding GFAP positive signals of a given field of view for each section was assessed by Image J analysis, n = 3–5 per group. Representative Western blot of EAAT2 (I) expression in Control (1), LPS (2) & LPS + HUCPVC (3) groups. Densitometric analysis of EAAT2 protein expression (J). Data normalized to GAPDH, n = 5–9 per group. *p < 0.05, **p < 0.005, ***p < 0.0005, one-way ANOVA with Tukey’s multiple comparison tests

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