Fig. 1

HUCPVC modulates LPS-induced activation of Kynurenine pathway enzymes

Schematic diagram of the Kynurenine pathway (A). mRNA expression of Kynurenine pathway enzymes after 24 h LPS and HUCPVC treatments (B-F). Total RNA from the brain was isolated from Control, LPS, and LPS + HUCPVC groups (n = 3 per group) and subjected to qPCR. Individual measurements were normalized using GAPDH as a housekeeping gene and the Fold change relative to Control was calculated by the ΔΔCt method. *p < 0.05, **p < 0.005, ***p < 0.0005, ****p < 0.0001, ns = not significant, one-way ANOVA with Tukey’s multiple comparison tests. TRY, tryptophan; ido, indoleamine dioxygenase; KYN, kynurenine; kat, kynurenine aminotransferase; kmo, kynurenine monooxygenase; 3HK, 3-hydroxykynurenine; 3HAA, 3-hydroxyanthranilic acid; haao, 3-hydroxyanthranilic acid dioxygenase; qprt, quinolinate phosphoribosyltransferase; NAD, Nicotinamide adenine dinucleotide