Fig. 6

Dio inhibits IL-1β-mediated activation of NF-κB signaling pathway. Chondrocytes were exposed to IL-1β (10 ng/mL) with or without Dio (200, 400, and 800 ng/mL) for 30 min. A Representative western blots and (B and C) quantitative analysis of P65, P-P65, IKK-β, P- IKKα/β, IκBα and P- IκBα in each group. D The nuclear translocation of P65 was observed by immunofluorescence after the cells treated with IL-1β (10 ng/mL) in absence or presence of Dio (800 ng/mL). ^#P < 0.05 vs. control group; ^*P < 0.05 vs. IL-1β group; ^**P < 0.01 vs. IL-1β group; n = 3