Fig. 4

LPS-induced pulmonary leakage was ameliorated by restoring junctional protein after CU06-1004 treatment. (A) Representative image of extravasation of Evans-blue dye into the lung and (B) quantitative results for Evans-blue dye, measured using spectrophotometry, absorbance at 620 nm. N = 8–10 per group. (C, D) Immunofluorescence staining images of intercellular junctions (green) in sections of lung, (C) adherent junction; VE-cadherin and (D) tight junction; ZO-1, with endothelial marker; CD31 (red), and DAPI (blue), confocal microscopy. Scale bar: 20 μm. (E, F) Quantitative results for (E) VE-cadherin and (F) ZO-1 expression assessed based on relative fluorescent intensity using ZEN software. N = 6–10 per group. Results presented as mean ± SEM values; * p < 0.05, ** < p 0.01, *** p < 0.001