Fig. 3

CU06-1004 reduced LPS-induced cytokine levels in bronchoalveolar lavage fluid (BALF), serum, and lung tissue. (A–C) BALF collected 32 h after LPS treatment. Inflammatory cytokines in BALF, including (A) TNF-α, (B) IL-6, and (C) IL-1β, measured using ELISA. (D–F) Cytokines in serum measured using ELISA, including (D) TNF-α, (E) IL-6, and (F) IL-1β, after whole-blood collection in the left ventricle. N = 4–6 per group. (G) Western blot analysis of TNF-α, IL-6, and IL-1β in mouse lung lysate; β-actin used as the loading control. (H) Quantitative graph of western blot analysis using ImageJ software as fold change. Results presented as mean ± SEM values; * p < 0.05, ** < p 0.01, *** p < 0.001