Fig. 3

Catecholamine production by RASFs. a Time-dependent synthesis of dopamine (DA), norepinephrine (NE) and epinephrine (EPI) by RASF. Extracellular levels of catecholamines after 2-, 6-, 12- and 24-hours incubation with complete medium are shown. b, c Extracellular (b) and intracellular (c) catecholamine levels after 72-hour inhibition of DDC with benserazide hydrochloride (BZD, 50 μM). d, e Extracellular (d) and intracellular (e) catecholamine levels after 24-hours inhibition of MAO and COMT with M30 (MAO inhibitor, 10 μM) and OR486 (COMT inhibitor, 10 μM). f, g Extracellular (f) and intracellular (g) catecholamine levels after 24-hours inhibition of VMAT with reserpine (RSP, 10 μM). h, i Extracellular (h) and intracellular (i) catecholamine levels after 24-hours inhibition of DAT and NET with indatraline hydrochloride (IDA, inhibitor of both DAT and NET, 10 μM). (n = 4–5) * p < 0.05, ** p < 0.01, *** p < 0.001, for comparisons between inhibitor treated and untreated groups by MANN-Whitney U test, and for comparisons between 2-hour-incubation group and the other three incubation time groups by one-way ANOVA with Tukey’s post-hoc test