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Fig. 1 | Journal of Inflammation

Fig. 1

From: Components of the sympathetic nervous system as targets to modulate inflammation – rheumatoid arthritis synovial fibroblasts as neuron-like cells?

Fig. 1

Expression of components of the catecholaminergic pathway in rheumatoid arthritis synovial fibroblasts (RASFs) under basal conditions and after stimulation with tumor necrosis factor (TNF). a-f Immunofluorescence (IF) and western blot (WB) images of dopamine transporter (DAT) (a), tyrosine hydroxylase (TH) (b), monoamine oxidase-A (MAO-A) (c), monoamine oxidase-B (MAO-B) (d), vesicular monoamine transporter 1 (VMAT1) (e) and vesicular monoamine transporter 2 (VMAT2) (f). Staining with respective antibody isotypes (upper left) served as negative control, target proteins are shown in green, cell nuclei (blue) were stained with DAPI (Bar 50 μm). Mouse brain homogenate was used as positive control (Ctl) in WB experiments. g-q Relative mRNA expression of components of the catecholaminergic pathway in RASFs with and without TNF stimulation for 6-, 12- and 24-hours including TH (g), DOPA decarboxylase (DDC) (h), dopamine-beta-hydroxylase (DBH) (i), phenylethanolamine N-methyltransferase (PNMT) (j), norepinephrine transporter (NET) (k), DAT (l), VMAT1 (m), VMAT2 (n), MAO-A (o), MAO-B (p) and Catechol-O-methyltransferase (COMT) (q). (n = 5) * p < 0.05, ** p < 0.01, comparing with the control group of each stimulation at a given time point by paired Student’s t-test. Black, control conditions; Red: stimulated with TNF (10 ng/mL); 6 h, 12 h, 24 h = stimulated time in hours

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