Fig. 3

TRPM2 mediates the MSU crystal-induced inflammation in murine air pouches. A Representative Hematoxylin and Eosin (H&E) staining of the air pouche membranes from of WT and TRPM2^−/− mice treated with MSU crystals. B The scatter plots show the inflammatory cell infiltration into the air pouche membranes of WT and TRPM2^−/− mice. Scale bars, 20 μm. Mean ± SD is displayed. p = 0.0065. C Representative staining by immunohistochemistry of MPO expression in air pouche membranes. Nuclear counterstain was performed with hematoxylin. MPO-expressing cells are brown in color. Scale bars, 20 μm. D Representative flow cytometry plots and E quantification of neutrophils (Ly6G⁺) in air pouches fluids of WT and TRPM2^−/− mice. Mean ± SEM is displayed. p = 0.0084. F Reduced concentrations of IL-1β, IL-6 and TNF-α in air pouches fluids from TRPM2^−/− mice. Mean ± SD is displayed. p < 0.0001