Skip to main content
Fig. 1 | Journal of Inflammation

Fig. 1

From: Transient receptor potential melastatin 2 regulates neutrophil extracellular traps formation and delays resolution of neutrophil-driven sterile inflammation

Fig. 1

TRPM2 is involved in MSU crystals-induced NET formation and aggregation. A Representative fluorescence microscopy images of neutrophils of WT or TRPM2−/− mice that had been treated with MSU crystals for 2 h. NETs were fixed and stained with DNA (DAPI), MPO and NE. B The scatter plots shows quantification of NETs in WT and TRPM2−/− neutrophils. Scale bars, 20 μm. p = 0.0005. C Representative NETs morphologyies, such as spiky (red), cloudy (green), spike-like (yellow) and bulky (blue) artificially stained from a blinded examiner. D The pie charts describe the classification of the NETs morphology. E The dynamic changes of the levels of extracellular DNA in the supernatants of neutrophils derived from WT and TRPM2−/− mice for 340 min (20 min intervals) after addition of MSU crystals. Mean ± SEM is displayed. The p-values at 220 min, 240 min, 260 min, 280 min, 300 min and 320 min are 0.0008, <0.0001, 0.0007, 0.0043, 0.0005 and 0.0010, respectively. F Representative photographs of aggNETs formed after injection of 5 mg MSU crystals into preformed air pouches in WT and TRPM2−/− mice. G Representative photographs of aggNETs formed after intraperitoneal (i.p.) injection of 20 mg MSU crystals into WT and TRPM2−/− mice that had been pretreated with thioglycolate 24 h before. H The scatter plots show the degree of NETs aggregation. Mean ± SD is displayed. p = 0.0020. I Levels of extracellular DNA in the supernatants of air pouches lavage fluid of WT and TRPM2−/− mice. Mean ± SEM is displayed; p = 0.0163

Back to article page