Table 1 Primers of RT-qPCR analysis (5’ → 3’)
Gene | Forward primer | Reverse primer |
|---|---|---|
IL-6 | 5’-ATGGCAATTCTGATTGTATG-3’ | 5ʼ-GACTCTGGCTTTGTCTTTCT-3’ |
TNF-α | 5’-CCAGACCCTCACACTCACAAA-3’ | 5ʼ-GGCTGACGGTGTGGGTGAG-3’ |
IL-1β | 5’-GGGATGATGACGACGACCTGCTA-3’ | 5ʼ-ACAGCACGAGGCATTTTTGT-3’ |
IFN-γ | 5’-GAGGAACTGGCAAAAGGACG-3’ | 5ʼ-AGGTGCGATTCGATGACACT-3’ |
Gapdh | 5’-GAGGAACTGGCAAAAGGACG-3’ | 5’-AGGTGCGATTCGATGACACT-3’ |