Fig. 8

β-FNAs effect on LPS-induced CXCL10, CCL2, IL-6, IL-1β, and TNF-α expression in the plasma of male and female C57BL/6J mice. Mice (n = 11–12/group) were injected (i.p.) with saline (control), LPS (0.83 mg/kg), LPS followed immediately by β-FNA treatment (50 mg/kg; i.p.; LPS + β-FNA), or LPS followed by β-FNA 10 h post-LPS (LPS + β-FNA 10 h). 24 h post-LPS, mice were terminated followed by plasma collection. Levels of CXCL10 (A), CCL2 (B), IL-6 (C), IL-1β (D), and TNF-α (E) of plasma were measured by ELISA. Data are reported as mean ± SEM. Two-way ANOVA indicated a significant main effect of treatment (p < 0.0001), sex (p < 0.0001), and interaction (p < 0.04) on CXCL10 levels in the plasma. Two-way ANOVA determined CCL2 had a significant main effect of sex (p < 0.0001), treatment (p < 0.0001), and interaction (p < 0.0001). Two-way ANOVA determined IL-6 had a significant main effect of sex (p < 0.0001) but not treatment (p = 0.14) and interaction (p = 0.06). Two-way ANOVA determined IL-1β had a significant main effect of sex (p < 0.0001) but not treatment (p = 0.28) or interaction (p = 0.07). Two-way ANOVA determined TNF-α had a significant main effect of sex (p < 0.04) and treatment (p < 0.0001) but not interaction (p = 0.08). Pairwise comparisons were assessed using a Fisher’s LSD test; * indicates p < 0.05 vs. saline group; # indicates p < 0.05 vs. LPS group; Δ indicates p < 0.05 vs. males LPS