Fig. 4

Effects of 5-methoxyflavone on LPS-mediated expression of pro-inflammatory cytokines in BEAS-2B cells. (A) ELISA assay was performed to determine the expression levels of IL-6, TNF-α, IL-8 and MCP-1 in the culture supernatant of LPS-stimulated BEAS-2B cells with or without 5-methoxyflavone treatment. (B) ELISA assay was performed to determine the expression levels of IL-6, TNF-α and MCP-1 in the culture supernatant of LPS-stimulated BEAS-2B cells treated with 5-methoxyflavone alone or in combination with Nrf2 inhibitor (brusatol). ^###P < 0.001 versus the control group; ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 versus the LPS group; ^δP < 0.05, ^δδP < 0.01 versus the 5-methoxyflavone treatment alone