Fig. 7

Citrullination landscape of THP-1 macrophages polarized to proinflammatory (M1) and anti-inflammatory phenotypes (M2). The identified and quantified citrullinated proteins were engaged in signalling by interleukins, neutrophil degranulation, programmed cell death, DNA and RNA processing, proteasomal degradation, platelet activation and VEGF signalling. A Normalized citrullinated peptide quantities of R115ILELDQFK (XRCC6) and B GGELVYTDSEAR3039DVFEK (PLEC) in THP-1 macrophages treated with LPS (M1 phenotype) or IL-4 (M2 phenotype) for 48 h in the presence of BB-Cl. C Enriched functional network analysis of citrullinated sites in THP-1 macrophages, as generated by PINE. D Normalized citrullinated peptide quantities of NNTQVLINCR47NNK (SNRPD2) and E CDSSPDSAEDVR143K (AHSG) in THP-1 macrophages treated with siPAD2 or scrambled siRNA control (siCON) and LPS (M1 phenotype). *p < 0.05; n = 5 or 6