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Fig. 5 | Journal of Inflammation

Fig. 5

From: Extracellular vesicles isolated from hyperuricemia patients might aggravate airway inflammation of COPD via senescence-associated pathway

Fig. 5

The pathology of COPD may be associated with mtDNA in EVs via a senescence related pathway. A Identification of senescence related protein including CDKN1A, CDKN2A, IL1A and 53BP1 in HBE treated with CSE and EVs from healthy controls and HUA patients was performed by western blot. B RT-PCR analysis of mtDNA content in PBMCs derived from COPD patients with or without HUA was compared using RT-PCR (unpaired t test, *P < 0.05). C IF microscopy analysis of CDKN1A and CDKN2A expression in HBE cells treated with CSE and subsequent EVs from patients with or without HUA(X20). Scale bars, 500 μm. Abbreviation: PBMC, peripheral blood mononuclear cell; CDKNI1A, cyclin-dependent kinase inhibitor 1A; CDKN2A, cyclin-dependent kinase inhibitor 2A; 53BP1, recombinant Tumor Protein p53 Binding Protein 1; IL-1A, Human IL-1 alpha protein; HBE, human bronchial epithelial; CSE, cigarette smoke extract; EVs, extracellular vesicles

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