Fig. 3

The comparison of inflammation status in lung tissue and peripheral blood between COPD patients with or without HUA. A HE staining detected alveolar cells morphology (X20) and MASSON staining of lung tissue (X20). Scale bars, 50 μm. B Quantification of cell counts according to HE staining and fibrotic area of difference groups by MASSON staining (X20). Scale bars, 50 μm. C Expression of inflammatory factors, IL-1β, IL-6, IL-8 and MUC1 were detected in lung by multiplex immunofluorescences(X20). Scale bars, 50 μm. The immunofluorescence quantification of mean fluorescence intensity was indicated in the bar plot. D Multiplex immunofluorescences of location of Antitrypsin and Elane in lung tissues(X20). Scale bars, 50 μm. E The level of IL-6, IL-8, Antitrypsin and Elane in plasma from two groups detected by ELISA (unpaired t test, *P < 0.05 and ****P < 0.001). Abbreviation: COPD, chronic obstruction pulmonary disease; HUA, hyperuricemia; HE, Hematoxylin & Eosin; IL-6, interleukin-6; IL-8, interleukin-8; IL-1β, interleukin-1 beta; DAPI, 4’,6-Diamidino-2’-phenylindol; MUC1, mucin 1