Fig. 2

IAV activates and fires airway inflammation in bronchial epithelial cell through NLRP3 inflammasome pathway. A NHBE (n = 3) and DHBE cells (n = 3) were incubated with IAV (MOI = 2) for 0, 1, 3, 6, 12, 24 h, respectively; cell lysates were subjected to immunoblotting with the indicated antibodies. CK17/19 is an indicator of epithelial cells; β-actin was used as a reference protein. B After IAV (MOI = 0, 0.5, 1.0, 2.0, 5.0, or 10.0) incubation with NHBE/DHBE cells for 24 h, MTT was used to detect cell viability. The normalized ratio of C pro-caspase1, D caspase1 p20, E NLRP3, F pro-IL-1β, G IL-1β, H ASC, I pro-IL-18 and J IL-18 expression to β-actin were measured at the above time points (*denote P < 0.05, **denotes P < 0.01, ***denotes P < 0.001). Data are presented as the mean ± SD of three independent experiments