Fig. 2

TRIP6 deficiency impairs inflammatory NF-κB signaling activity in a DSS-induced model of experimental murine colitis. a Western blotting was used to assess the expression of TRIP6 in colon tissues from TRIP6^+/+ mice following DSS treatment, with representative images being shown. b Western blotting was used to assess the expression of IκBα in colon tissues from TRIP6^+/+ and TRIP6^−/− mice following DSS treatment, with representative images and being shown (left). Protein levels of IκBα were quantified by using densitometry and normalized to those of β-actin for comparison (Right). Data are means ± SD. n = 3, *p < 0.05 and **p < 0.01. c-d qPCR (c) and ELISAs (d) were respectively used to evaluate TNFα and IL-6 mRNA and protein levels in colon tissues. e ELISAs were used to evaluate serum TNFα and IL-6 levels in TRIP6^+/+ and TRIP6^−/− mice over the course of DSS induction and recovery. Data are means ± SD. **p < 0.01; **p < 0.01; ***p < 0.001; ns, not significant