Fig. 5

Gain of miR-200a-3p partially reversed Rg3-induced mitochondrial dysfunction improvement in LPS-treated hepatocytes. Hepatocytes were divided into control LPS, LPS + Rg3, LPS + Rg3 combined with miR-NC transfection, or LPS + Rg3 combined with miR-200a-3p mimic transfection. (A-B) Western blot assay for the protein levels of LC3, P62, and Beclin-1 in treated cells. (C) MitoSOX™ Red staining for the mitochondrial ROS level in treated cells. (D-E) JC-1 staining for the MTP level in treated cells. (F-G) Western blot assay for the protein levels of complex I, complex II, and OPA1 in treated cells. n = 3 independent experiments. Differences between groups were compared using the Mann–Whitney U test or Kruskal–Wallis-Wallis test. *P < 0.05, **P < 0.01