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Fig. 3 | Journal of Inflammation

Fig. 3

From: Ginsenoside Rg3 alleviates septic liver injury by regulating the lncRNA TUG1/miR-200c-3p/SIRT1 axis

Fig. 3

TUG1 sponged miR-200a-3p to activate the SIRT1/AMPK pathway. (A) Binding position between TUG1 and miR-200a-3p predicted by starBase. (B) Dual-luciferase reporter assay for the luciferase density of TUG1-WT and TUG1-MUT in hepatocytes cotransfected with miR-NC or miR-200a-3p mimic. (C) RIP assay for the binding potency between TUG1 and miR-200a-3p. (D-F) Hepatocytes were divided into control, LPS, LPS + Rg3, LPS + Rg3 combined with sh-NC transfection, or LPS + Rg3 combined with sh-TUG1 transfection. (D) qRT–PCR assay for the relative expression of miR-200a-3p in treated cells. (E-F) Western blot assay for the protein levels of SIRT1, p-AMPK, AMPK, p-ACC, and ACC in treated cells. (G) Binding position between miR-200a-3p and SIRT1 predicted by starBase. (H) Dual-luciferase reporter assay for the luciferase density of SIRT1-WT and SIRT1-MUT in hepatocytes cotransfected with miR-NC or miR-200a-3p mimic. (I) qRT–PCR assay for the relative expression of TUG1, miR-200a-3p, and SIRT1 in hepatocytes transfected with pcDNA3.1-NC or pcDNA3.1-TUG1. (J) qRT–PCR assay for the relative expression of miR-200a-3p and SIRT1 in hepatocytes transfected with miR-NC or miR-200a-3p mimic. (K-L) Western blot assay for the protein levels of SIRT1, p-AMPK, AMPK, p-ACC, and ACC in hepatocytes divided into control, LPS, LPS combined with pcDNA3.1-NC transfection, LPS combined with pcDNA3.1-TUG1 transfection, LPS combined with pcDNA3.1-TUG1 + miR-NC transfection, or LPS combined with pcDNA3.1-TUG1 + miR-200a-3p mimic transfection. n = 3 independent experiments. Differences between groups were compared using the Mann–Whitney U test or Kruskal–Wallis-Wallis test. *P < 0.05, **P < 0.01

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