Fig. 5

MiR-223 mediates oxidative stress and JNK signalling pathway activation by regulating HMGB2 expression, thereby affecting LPS-induced autophagy of alveolar epithelial cells. A549 cells were simultaneously transfected with miR-223 mimics and HMGB2 overexpression vectors and then incubated with LPS to induce injury. A The miR-223 expression in the cells was detected by RT-qPCR. B RT-qPCR was used to detect the mRNA expression of HMGB2. C Protein expression levels HMGB2, JNK, p-JNK, and β-actin in the cells were detected by western blot. D The apoptotic rate was assessed by flow cytometry and its quantification data. E The fluorescent probe detected ROS level in the cells. F ELISA was used to detect the concentration of 8-OHdG in cells. G Western blot analysis of the autophagy-related proteins in the cells. H The concentrations of TNF-α, IL-1β and IL-6 were assessed by ELISA. n=3. *P < 0.05, ** P < 0.01, *** P < 0.001