Fig. 3

MiR-223 directly binds HMGB2 and regulates its expression. A RT-qPCR detected the gene expression of HMGB2 in the LPS-treated A549 cells and the untreated control cells. B Western blot analysis of HMGB2, JNK, p-JNK and β-actin in the LPS-treated A549 cells. C The effect of miR-223 overexpression plus LPS treatment or only LPS treatment on HMGB2 mRNA expression. D The expression of HMGB2, JNK, p-JNK and β-actin proteins in A549 cells following treatment with LPS in the presence or absence of miR-223 overexpression was detected by western blot. E Online prediction of miR-223 and HMGB2 binding sites. F The A549 cells were co-transfected with miR-223 mimics or a miR-223 inhibitor. Then, luciferase activity was detected. n=3. *P < 0.05, ** P < 0.01, *** P < 0.001