Fig. 2

The miR-223 suppresses oxidative stress and autophagy in LPS-treated alveolar epithelial cells. A RT-qPCR detection of the miR-223 expression in LPS-treated A549 cells. B After A549 cells were transfected with the miR-223 mimic, the cells were further induced by LPS. The miR-223 expression was detected by RT-qPCR. C The effect of miR-223 overexpression plus LPS treatment or LPC treatment on A549 cell apoptosis was assessed by flow cytometry. D The fluorescent probe was used to detect ROS level in A549 cells following treatment with LPS in the presence or absence of miR-223 overexpression. E The 8-OHdG concentration in A549 cells following treatment of LPS in the presence or absence of miR-223 overexpression was assessed by ELISA. F Western blot analyses of the autophagy-related proteins in A549 cells following treatment with LPS in the presence or absence of miR-223 overexpression. G The concentrations of TNF-α, IL-1β and IL-6 in A549 cells following LPS treatment in the presence or absence of miR-223 overexpression were assessed by ELISA. n=3. *P < 0.05, ** P < 0.01, *** P < 0.001