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Fig. 6 | Journal of Inflammation

Fig. 6

From: USP10 alleviates sepsis-induced acute kidney injury by regulating Sirt6-mediated Nrf2/ARE signaling pathway

Fig. 6

USP10 promotes activation of the NRF2/HO-1 pathway through SIRT6 and attenuates LPS-induced renal tubular epithelial cell injury. (A-B) Western blot images show the expression levels of Sirt6, Nrf2 and HO-1 in control, LPS, LPS + Ad-GFP, LPS + Ad-Sirt6, Ad-GFP + shNC, Ad-USP10 + shNC and Ad-USP10 + shSirt6 HK-2 cells, **p < 0.01. GAPDH was used as an internal control. (C) MTT assay demonstrating cell viability in Ad-GFP + shNC, Ad-USP10 + shNC and Ad-USP10 + shSirt6 HK-2 cells, *p < 0.05. (D) Flow cytometry charts showing cell apoptosis in Ad-GFP + shNC, Ad-USP10 + shNC and Ad-USP10 + shSirt6 HK-2 cells, **p < 0.01. (E) Comparison of MDA and SOD levels in Ad-GFP + shNC, Ad-USP10 + shNC and Ad-USP10 + shSirt6 HK-2 cells using the colorimetric kits, *p < 0.05, **p < 0.01

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Journal of Inflammation

ISSN: 1476-9255

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