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Fig. 6 | Journal of Inflammation

Fig. 6

From: Bullatacin triggers immunogenic cell death of colon cancer cells by activating endoplasmic reticulum chaperones

Fig. 6

Bullatacin triggers ICD via activation of the ERS signalling pathway. A, B Real-time PCR analyses of the mRNA expression of calnexin and CHOP in two colon cancer cell lines treated with bullatacin at the indicated concentrations. Significance: **P < 0.01 versus control. C, D Western blot analyses of the cell membrane protein expression of CRT and HSP90 in SW480 and HT-29 cells treated with bullatacin for the indicated times. The relative protein expression of CRT and HSP90 was evaluated by quantifying the greyscale values with ImageJ. Significance: **P < 0.01 versus control. Western blot analyses of the protein expression of IRE1, pIRE1, cleaved-ATF6 and ATF6 in SW480 and HT-29 cells treated with bullatacin for 12 h. The relative protein expression of IRE1, pIRE1, cleaved-ATF6 and ATF6 was evaluated by quantifying the greyscale values with ImageJ. Significance: **P < 0.01 versus control

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