Fig. 4

TUG1 mediated miR-194/SIRT1 axis in hepatocytes. a Bioinformatics analysis was used to predict the bind sites between miR-194 and TUG1 or SIRT1. b The relative luciferase activity was tested by dual-luciferase reporter gene assay. WRL-68 cells were pre-treated with 1 nm DEX for 1 h and then cultured in OGD medium. c The expression of miR-194 in WRL-68 cells was assessed by qPCR. d The expressions of miR-194 and SIRT1 in WRL-68 cells were assessed by qPCR. e The protein expression of SIRT1 in WRL-68 cells was tested by western blotting. The relative protein expressions were quantified via normalizing to GAPDH. *p < 0.05, ** p < 0.01 and *** p < 0.001