Fig. 7

Nuclear-to-cytoplasmic translocation of AIM2 and increased cleaved IL-1β in bronchiolar epithelium of cigarette smoke-exposed mice. a, b Representative confocal images of AIM2 (red, Ab #1, aa250–300/354) and cleaved IL-1β (green) in bronchioles of mice exposed to cigarette smoke for 24 week (b), vs. sham control (a). c, d Line profile analysis revealing nuclear localization of AIM2 in control mice (c) and cytoplasmic in smoked mice (d). Pixels intensities measured along the line (inset) were plotted in y-axis, vs. their localization in x-axis. e, f Quantitation of AIM2 cytoplasmic specks and cleaved IL-1β in mouse bronchioles, cigarette smoke-exposed (n = 4) vs. sham control (n = 4)