Fig. 5

MiR-494-3p targeted to E-cadherin. a: The predicted binding site sequences of E-cadherin and miR-494-3p. b: The relative luciferase activities was assessed by luciferase reporter assay. c, d: The relative expression levels of miR-494-3p and E-cadherin mRNA were detected by qRT-PCR. e: The protein level of E-cadherin was measured by western blot. Results were presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001