Fig. 5

Effects of NF-κB inhibition on over-mechanical stretching stress induced inflammation in rat nucleus pulposus cells. ELISA was used to measure the levels of TNF-α (a) and IL-1β (b). Real-time PCR was used to measure the mRNA expressions of TNF-α (c) and IL-1β (d). e Western blot analysis for the protein expression of TNF-α and IL-1β. GAPDH was used as loading control. The relative expressions were normalized to control (f). Data are presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001 between the indicated groups