Fig. 3

Effects of NF-κB inhibition on over-mechanical stretching stress induced apoptosis of rat nucleus pulposus cells. CCK-8 was used to measure the cell viability (a). Apoptotic cells were stained with Annexin V-PE and PI, and analyzed by flow cytometry (b and c). d Western blot analysis for the protein expression of Bax and Bcl-2. GAPDH was used as loading control. The relative expressions of Bax/Bco-2 were calculated (e). Data are presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001 between the indicated groups