Fig. 4

MAR1 suppressed inflammatory response in LPS-induced RAW 264.7 cells and hPBMCs via the SIRT1/PGC-1α/PPAR-γ pathway. Cells were treated with LPS (10 µg/ mL) for 24 h or treated with 10 nM of MAR1 for 12 h before LPS treatment. a, TNF-α, IL-1β and IL-6 expression were detected by RT-qPCR; b, TNF-α, IL-1β and IL-6 secretion were measured using ELISA. c-d, The mRNA and protein levels of SIRT1, PGC-1α and PPAR-γ were detected by RT-qPCR and Western blot, respectively. Data were shown as the means ± SD. Each experiment was repeated in triplicate. *p < 0.05, **p < 0.01, ***p < 0.001