Fig. 5

Overexpression of SP1 attenuated renal ischemia-reperfusion injury in rats. A rat model of renal ischemia-reperfusion injury was prepared and treated with a SP1 overexpression vector. a Detection of Scr levels by ELISA. b RT-qPCR was used to detect the expression of miR-205. c TUNEL staining was used to detect cell apoptosis. d Immunohistochemical detection of SP1 expression distribution. e Western blot detected the expression of PTEN, AKT, p-AKT, Beclin-1, p62, LC3II and LC31. The data are expressed as the mean ± SD and are representative of 3 experiments. *P < 0.05, **P < 0.01, ***P < 0.001