Fig. 4

Overexpression of SP1 protected HK-2 cells injury induced by anoxia-reoxygenation via miR-205/PTEN/Akt pathway mediated autophagy. The HK-2 cells were transfectd with SP1 overexpression and miR-205 inhibitor. a-b The expression of SP1 and PTEN was detected RT-qPCR. c Western blot detection of PTEN, AKT, p-AKT, Beclin-1, p62,LC3II and LC31. d Cell proliferation measured by MTT assay. e Flow cytometry analysis of cell apotosis. f-g The secretion of IL-6 and TNF-α were detected by ELISA. The data are expressed as the mean ± SD and are representative of 3 experiments. *P < 0.05, **P < 0.01, ***P < 0.001