Fig. 3

PTEN was a direct target of miR-205. The HK-2 cells were transfectd with miR-205 inhibitor or mimic. a-b The expression of miR-205 and PTEN was detected by RT-qPCR c The expression of PTEN was detected by RT-qPCR and western blot. d-e PTEN was a direct downstream target of miR-205 was confirmed by both bioinformatics target gene prediction and dual-luciferase report assay. The data are expressed as the mean ± SD and are representative of 3 experiments. *P < 0.05, **P < 0.01, ***P < 0.001