Specific lysis of target cells in vitro. GLEA2-specific cytotoxic T lymphocytes (CTLs) were elicited in vitro by weekly stimulation of peripheral blood lymphocytes with irradiated autologous DCs transduced with Ad-GLEA2. GLEA2-specific CTLs were tested against U251 cells. CTLs generated from Ad-LacZ transduced DCs and CTLs generated from non-treated DCs were used as controls. Triplicate experiments showed consistent results. Compared with controls,*P < 0.05.