Fig. 4

Immunohistochemical staining of iNOS in the paw tissue. a Paw tissue sections were immunostained for iNOS at 6 and 24 h in the saline, CRG, and CRG + US groups. Arrows indicate representative iNOS positive cells. b The expression levels of iNOS are quantified by ImageJ. The graph shows relative amount of iNOS expression to that of the saline group. Data were presented as mean ± S.E. of 3 independent experiments (n = 3). Statistical comparison was conducted on the saline versus CRG groups and the CRG versus CRG + US groups. *P < 0.05, ** P < 0.01, and *** P < 0.001. Scale bar: 100 μm