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Fig. 3 | Journal of Inflammation

Fig. 3

From: Circular RNA-CDR1as acts as the sponge of microRNA-641 to promote osteoarthritis progression

Fig. 3

CircRNA-CDR1as interacts with miR-641. a circRNA-CDR1as contains 19 sites complementary to miR-641, as analyzed by the bioinformatics program starBase. b Detection of miR-641 by qRT-PCR. c Expression levels of miR-641 were assessed after knocking down circRNA-CDR1as. d Following transfection with p-circRNA CDR1as, the expression levels of miR-641 were detected using qRT-PCR. e miR-641was pulled down and enriched with circRNA-CDR1as specific probe and then detected by qRT-PCR. f The biotinylated miR-641 or its control is transfected into chondrocytes. After streptavidin capture, circRNA-CDR1as mRNA levels are quantified by qRT-PCR. Data are expressed as mean + SEM. *p < 0.05 (t-test). Shown are the representative results from at least 3 independent experiments

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