Table 1 Primers of mRNA, lncRNA, and miRNA
Name | sequence(5′-3′) |
|---|---|
H-IL-6-F | ACTCACCTCTTCAGAACGAATTG |
H-IL-6-R | CCATCTTTGGAAGGTTCAGGTTG |
H-IL-8-F | GACCACACTGCGCCAACAC |
H-IL-8-R | CTTCTCCACAACCCTCTGCAC |
TNF-α-F | CTGCACTTTGGAGTGATCGG |
TNF-α-R | GCTTGAGGGTTTGCTACAACAT |
H-Iκκβ-F | CGATGGCACAATCAGGAAACAGGT |
H-Iκκβ-R | ATTGGGGTGGGTCAGCCTTCTC |
H-IKB-α-F | ACACCTTGCCTGTGAGCAGG |
H-IKB-α-R | AGCACCCAAGGACACCAAAA |
β-Actin-F | CATGTACGTTGCTATCCAGGC |
β-Actin-R | CTCCTTAATGTCACGCACGAT |
H-lncMALAT1-F | AAAGTCCGCCATTTTGCCAC |
H-lncMALAT1-R | ACAACTCGCATCACCGGAAT |
H-lncHotair-F | GCAGTGGGGAACTCTGACTC |
H-lncHotair-R | TTGAGAGCACCTCCGGGATA |
H-lncH19-F | GACATCTGGAGTCTGGCAGG |
H-lncH19-R | CTGCCACGTCCTGTAACCAA |
H-lncNeat1-F | GGCAGGTCTAGTTTGGGCAT |
H-lncNeat1-R | CCTCATCCCTCCCAGTACCA |
hsa-mir-346-RT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCCGCTC |
hsa-mir-346-F | GGTCTCTGTGTTGGGCGTCT |
hsa-miR-18a-3p-RT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCCAGAA |
hsa-miR-18a-3p-F | ACTGCCCTAAGTGCTCCTT |
hsa-mir-324-3p-RT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCCAGCA |
hsa-mir-324-3p -F | CCCACTGCCCCAGGTGCT |
hsa-miR-146b-5p-RT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACACCAGA |
hsa-miR-146b-5p-F | GCCCTGTGGACTCAGTT |
hsa-miR-18b-5p-RT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCTAACT |
hsa-miR-18b-5p-F | GCGTAAGGTGCATCTAGTGCAG |
hsa-mir-19a-RT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTCAGTT |
hsa-mir-19a-F | TGTGCAAATCTATGCAAA |
hsa-miR-19b-3p-RT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTCAGTT |
hsa-miR-19b-3p-F | GTGTGCAAATCCATGCAA |
Universe-R | GTGCAGGGTCCGAGGT |
U6-F | CGCTTCGGCAGCACATATAC |
U6-R | CGAATTTGCGTGTCATCCTTG |