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Fig. 6 | Journal of Inflammation

Fig. 6

From: Dexamethasone can attenuate the pulmonary inflammatory response via regulation of the lncH19/miR-324-3p cascade

Fig. 6

The attenuation of lncH19 by miR-324-3p was inhibited as the inflammatory response attenuated under treatment with IL-1β (10 ng/mL) and Dex (1 μM) at the same time. a. Levels of the candidate miRNAs, as determined via RT-PCR, for examining the performance of the miRNA inhibitor. b. Cell proliferation was determined using the MTS assay. c. Cell apoptosis was determined via flow cytometry. d. mRNA levels of the inflammatory cytokines. e. ELISA for detecting the levels of the inflammatory cytokines. f. Western blot for detecting the levels of the inflammatory genes. Data are presented as mean ± SD. N = 3. *P < 0.05, **P < 0.01. Dex: dexamethasone. IL-1β: interleukin-1 β; NC + miRNA NC: shNC + IL-1β + Dex + miRNA NC; NC + miR-324-3p inhibitor: shNC + IL-1β + Dex + miR-324-3p inhibitor; shlncH19 + miRNA NC: shlncH19 + IL-1β + Dex + miRNA NC; shlncH19 + miR-324-3p inhibitor: shlncH19 + IL-1β + Dex + miR-324-3p inhibitor

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