Fig. 1

a pulmonary inflammatory cell model was established by inducing A549 cells with IL-1β (10 ng/mL) or LPS (1 μg/mL) for different time periods. IL-1β proved to be a more robust inflammatory cell model. A. RT-PCR determination of the corresponding mRNA levels of the inflammatory genes of IKβ-α, IKKβ, IL-6, IL-8, and TNF-α; GAPDH was used as the housekeeping gene. b. Levels of the inflammatory cytokines IL-6, IL-8, and TNF-α were determined via ELISA. Data are presented as mean ± SD N = 3. **P < 0.01 vs. A549 0 h. IL-1β: interleukin-1β, LPS: lipopolysaccharide