Table 1 Sequence of primers and annealing temperature
Gene | Primer sequence | Annealing temperature |
|---|---|---|
Hepcidin | F 5′- AGATGGCACTAAGCACTCGG − 3′ R 5′- ATCAGCAGCGCACTGTCATC − 3’ | 56 °C |
β-actin | F 5’- GAAATCGTGCGTGACATTAAAGAG − 3′ R 5′- GCGGCAGTGGCCATCTC − 3’ | 56 °C |