Fig. 4

THRIL regulated miR-19a/TNF-α axis to promote the apoptosis of HBEpCs. HBEpCs cultivated in medium supplemented with 0, 2, 5 or 10 μg/ml LPS for 24 h, followed by the measurement of the expression levels of THRIL by performing RT-qPCR (a). The effects of THRIL, miR-19a and TNF-αon the apoptosis of HBEpCs were analyzed by cell apoptosis assay after the treatment with 10 μg/ml LPS for 48 h (b). All experiments were repeated 3 times and mean ± SD values were presented. *, p < 0.05. (Note: C: untransfected control cells; siTHRIL: THRIL siRNA)