Fig. 2

Comparison of writhing number in 20 min, writhing latency, and tail-flick latency of mice among groups. The mice were pretreated with saline, or estradiol (0.2 mg/ml) and oxytocin (2 U/ml) to build a PD model, or MCC950 (2 mg/ml, an NLRP3 inhibitor) in the presence of estradiol and oxytocin. All tests were double-blinded. a the typical writhing response corresponds to the writhing number. b time from intraperitoneal injection of oxytocin to the first writhing reaction. c One thirdx of the tail was placed in hot water at 52 °C, and the time was measured from the tail entering the water to exiting. Data are expressed as means ± SD (n = 10). *P < 0.05, **P < 0.01 compared to the PD group