Fig. 2

Neutrophil infiltration in the airway of βENaC-overexpressing mice. (A) Representative confocal images of neutrophils, identified by high expression of neutrophil elastase (red) and myeloperoxidase (green). Shown are neutrophils that are intravascular (a), attached to the vascular inner wall (b), and extravasated (c). (B-D) Representative confocal images of neutrophils identified by high expression of myeloperoxidase (green) and lobular shape of nuclei (Blue, DAPI). Shown are neutrophils that are intravascular in a wild type control (B), in submucosal tissue near a relatively intact bronchiole of a βENaC animal (C), and infiltrating a mucus plug in a βENaC animal (D). Scale bars are in micrometers