Fig. 5

MiR-494 promoted macrophage M1 polarization and enhanced inflammatory damage via Nrdp1. a Detection of the inhibition efficiency of siRNAs against Nrdp1. Nrdp1 siRNA significantly attenuated Nrdp1 mRNA and protein levels. However, scramble siRNA did not attenuate Nrdp1 levels. b Macrophages were transfected with scramble siRNA or Nrdp1 siRNA, and then were treated with PBS or erythrocyte lysates for 3 days. After then, neurons were treated with conditioned medium from treated macrophage. Inhibition of Nrdp1 significantly promoted macrophage M1 polarization. c Intracerebroventricular injection of scramble siRNA or Nrdp1 siRNA was administered 10 min after ICH. Inhibition of Nrdp1 significantly attenuated neuron viability in vitro. d After 3 days of ICH, the cerebral water content of mice (n = 10) was also analyzed. In addition, the neurological deficit tests were performed by behavioral measurement. Inhibition of Nrdp1 significantly enhanced the cerebral water content and promoted neurological damage. Experiments performed in triplicate showed consistent results. The differences were analyzed using ANOVA. *P < 0.05