Fig. 3

MiR-494 promoted inflammatory injury in vitro and in vivo. a Macrophages were transduced with miR-494 mimics or inhibitors, and then were treated with PBS or erythrocyte lysates for 3 days. The neurons were cocultured with a mixture of macrophage conditioned medium. Administration of miR-494 mimics significantly enhanced macrophage toxicity to neuron, while administration of miR-494 inhibitors significantly inhibited macrophage toxicity to neuron. b-c Intracerebroventricular injection of miR-494 mimics or inhibitors was administered 10 min after ICH. MiR-494 mimics significantly promoted water content and neurological damage, while miR-494 inhibitors significantly decreased water, content and neurological damage. Experiments performed in triplicate showed consistent results. The differences were analyzed using ANOVA. *P < 0.05