Fig. 5
In vivo induction of epitope-specific CTLs in vivo. 12 weeks old HLA-A*0201/Kb mice were immunized three times once a week, by subcutaneous injection in the back with 1 × 106 syngeneic mature DC pulsed with the peptides. After 7 days, the spleens of the mice were removed and the splenocytes were harvested as effectors. The cytotoxic activities of CTLs were determined against target cells at various E/T ratios using 51 Cr release assay. Experiments performed in triplicate showed consistent results. Compared with controls, *P < 0.05