Fig. 4From: Prediction and identification of novel HLA-A*0201-restricted cytotoxic T lymphocyte epitopes from endocanAntibody inhibition assay by anti-HLA-A2 or anti-CD8 antibody. Peptide-coated T2 target cells were incubated with or without anti-HLA-A2 antibody from BB7.2 cells for 1 h at 4 °C. Moreover, effectors induced by different endocan -derived peptides were also incubated with or without anti-CD8 antibody for 1 h at 4 °C. The cytotoxic activities of CTLs against T2 cells were analyzed at various E/T ratios by 51 Cr release assay. Experiments performed in triplicate showed consistent results. Compared with control, *P < 0.05Back to article page