Fig. 1

Endocan expression in different target cells. The tumor cells were homogenized, and total RNA was isolated using Tripure Isolation Regent Kit. Two microliters RT product was amplified with PCR by using TaqDNA polymerase (using standard procedures). RT-PCR products were then run on a gel and visualized with ethidium bromide. For Western blot analysis, proteins in the cell extracts were separated by SDS-PAGE and were then analyzed with anti- endocan MAb. 1: U251 cells; 2: BV2 cells; 3: autologous lymphocytes